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Metabolism of dietary fat in relation to risk factors for coronary heart disease. - AN0221

Description
We hypothesise that the fateof dietary fatty acids during initial hydrolysis of chylomicron-triacylgylcerol (TG) depands on thier nature and their positional distribution: saturated fatty acids, particularly if present in the sn-2 position, will tend to escape immediate tissue uptake. They may then affect hepatic metabolism and ultimately plasma cholestorol concentrations. We will test this by feeding TGs of known structure and examining the fate of individual fatty acids during passage through adipose tissue in vivo. At the same time we will examine the hypothesis that the action of lipoprotein lipase (particulary on saturated fats) is intimately linked with the activation of the coagulation system. In related studies we will examine the effects of different fats on sensitivity to insulin, testing the hypothesis that any effects oberserved may be mediated through changes in body fat distribution. We hyopthesise that the fate of dietary fatty acids during initial hydrolysis of chylomicron-triacylglycerol (TG) depends on their nature and their positional distribution: saturated fatty acids, particularly if present in the sn-2 position will tend to escape immediated tissue uptake. They may then affect hepatic metabolism and ultimately plasma cholesterol concentrations. we will test this by feeding TGs of known structure and examining the fate of individual fatty acids during passage through adipose tissue in vivo. At the same time we will examine the hypothesis that the action of lipoprotein lipase is intimately linked with activation of the coagulation system. In related studies we will examine the effects of different fats on sensitivity to insulin, testing the hypothesis that any effects observed may be mediated through changes in body fat distribution.
Objective
1. To elucidate the initial metabolic handling of dietary triacylglycerols(TG) of known structure, during passage of chylomicron-TG through adipose tissue : the fate of individual fatty acids should be elucidated in terms of tissue uptake, release into plasma, and possibly incorporation into VLDL (TG or cholesteryl-ester). 2. To elucidate the relationships between the metabolism of ingested fat and activation of the coagulation system. 3. To elucidate the effects of feeding different types of fat on sensitivity to insulin, and to test the hypothesis that any effects observed may be mediated through changes in body fat distribution.
Time-Scale and Cost
From: 1994

To: 1997

Cost: £470,559
Contractor / Funded Organisations
Oxford Lipid Metabolism Group
Keywords