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Studies of the effects of structure of triacylglycerols on their absorption and metabolic disposition in man. - AN0218

Project aims to test the dependence of digestibility, absorption and metabolism of FA substituents in TAG upon: stereo-chemical position; chain length; whether saturated or unsaturated and the degree of unsaturation of the other substituents. The project will synthesise structural TAG containing sn-1, 2 or 3 13C-labelled fatty acids, using palm oil (mainly saturated) and fish oil as starting materials. The 13C substituents will be one of: stearate(18:0); linoleate (18:2) or octanoate (8:0). We will measure the digestion, absorption and metabolism of the 13C-TAGs delivered by nasogastric infusion in healthy subjects. Concentrations of 13C-monoglycerides, diglycerides and RA in duodenal and jejunal fluid and appearance of 13C-Fas in chylomicrons, blood cell membranes and VLDL will be determined using TLC, HPLC, combustion IRMS and GCMS; oxidation will be measured as appearance of breath 13CO2. Psitional information on TAG of chylomicrons and VLDL will be obtained by stereospecific analysis.
1. To produce a stable-isotope labelled triacylglycerol at the rate of one compund every 4-8 weeks for use in physiological studies. 2. To test the compounds in normal healthy volunteers at the rate of 1-2 subjects studies per week. 3. To analyse samples as they are obtained. 4. To determine the effect of position of acylation, chain length, presence of unsaturation and "background" fatty acid content on TAG digestion, absorption and metabolism. 5. To discover what factors modify incorporation into chylomicron and VLDL TAG, VLDL phosphopolipid, cholesteryl ester, CO2 and adipose tissue phospholipids and possibly TAG.
Time-Scale and Cost
From: 1994

To: 1997

Cost: £357,658
Contractor / Funded Organisations
University - Dundee