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The effect of triglyceride structure on lipid and lipoprotein metabolism. - AN0217

Lipoprotein metabolism within the body is dominated by the action of two lipases, lipoprotein lipase and hepatic lipase. In order to provide precise information on how the activity of these two enzymes is affected by dietary lipid, a primary requirement is to have a reliable source of these two human enzymes. The cloning and over expression of these two enzymes in sf9 insect cells will provide the required source of pure enzyme for future work in this area. Lipoproteins can be modelled as emulsion particles and the hydrolysis of defined triglyceride in the particles by lipoprotein lipases can be measured using a unique continous fluorescence assay that is very sensitive and uses normal triglycerides as substrate. This work will be initiated using non-human lipoprotein lipases in oreder to provide basic information on these systems but will utilise human enzymes when available.
1. To provide an in vitro system that will enable large amounts of human lipoprotein lipase and human hepatic lipase to be produced at minimum cost so that these crucial enzymes are readily available for use in the study of human lipoprotein metabolism. 2. To provide fundamental data on lipoprotein metabolism that will allow the affects of the composition of dietary fat on human lipoprotein metabolism to be predicted. This information will allow realistic assessments to be made of the effect of changing dietary fat intake on human health.
Time-Scale and Cost
From: 1995

To: 1998

Cost: £249,967
Contractor / Funded Organisations
University - Southampton