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Development and validation of rapid viable pathogen testing and enumeration at low levels by a simple & low cost method. - FS1213

Description
New specific and highly sensitive liposome-amplified detection will be further developed at CAMR for rapidly detecting viable pathogens. It will be implemented and critically evaluated at LGC as a microbiologically-compatible contact gel for testing and enumerating viable organisms grown at low numbers, where the presumptive isolates rapidly detected can be grown on into colonies for established microbiology and confirmation of the rapid method in the expert laboratory. The investigation will make use of rapid ultrasonic extraction of organisms using the detection gel to collect the ultrasonically swept organisms.
Objective
1. To develop sensitive and specific detection gel responding directly to the viability and antigenicity of selected model pathogens in food poisoning. 2.
Time-Scale and Cost
From: 1993

To: 1996

Cost: £244,637
Contractor / Funded Organisations
Health Protection Agency
Keywords