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Rapid extraction and detection of specific pathogens from food by magnetic fluorescence amplification & flow cytometry - FS1206

Description
It has already been demonstrated in the research laboratory that flow cytometry has impressive potential as a 'rapid method' for the detection of specific bacteria. It is proposed to develop the novel concept of magnetic fluorescence amplification to increase the efficiency of extraction of bacteria from foods and, at the same time, to enhance the sensitivity of their detection by flow cytometry. This should result in an assay technique which is applicable to a wide range of foodstuffs and a reduction in the cost and complexity of the instrumentation required.
Objective
1. To develop a method to immobilise antibodies on super-paramagnetic particles. 2. To investigate the ability of these particles to attach to and recover bacteria from solution on application of a magnetic field. 3. To develop a method to load antibody =labelled particles with high quantities of fluorescent dye. 4. To assess the increas in fluorescence obtained with flow cytometry from particle-labelled bacteria compared to a representative conventional labelling scheme. 5. To apply the technique developed to detect pathogenic bacteria in some example food systems. 6. To assess the robustness of the new technique with commercial instrumentation.
Time-Scale and Cost
From: 1993

To: 1996

Cost: £302,530
Contractor / Funded Organisations
IFR - Institute of Food Research (BBSRC)
Keywords