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Large volume culture of toxic dinoflagellates - FC0720

R&D Summary Objectives:- Help establish a reliable supply of toxin-producing algae for use in the programme of research into toxic algae, including developing non-mouse tests. Key customer purpose:- Make toxins freely available to the research effort including the development of a non-animal, low cost test for toxins in shellfish and thus help fulfil testing requirements arising from new EC Directive on Shellfish Hygiene.
Element 1:i) Scale up present batch culture methods to provide sufficient toxic material for use in elements 2 and 3 (tritiated OA production) and for Torry RS and various research groups supported by MAFF. ii) Continue efforts to obtain unialgal cultures of UK. strains of P. lima. This work would be undertaken by the Culture Centre of Algae and Protozoa at DML. If successful, screen these for DSP toxin content. iii) Obtain other PSP strains of dinoflagellates for culturing and analysis pending the availability of UK. strains (to be supplied by Dr Jane Lewis, University of Westminster [separate MAFF commission]). Element 2:Establish the basic lipid profile of Prorocentrum spp. (phospholipids, triacylglycerols, sterols etc.) as a first step towards understanding the biosynthetic pathway of the compound. Confirmation of chemical structures by mass spectrometry, if necessary, would be done by buying time on a suitable instrument. This element would form the groundwork for future work which would probably involve university collaborators. Element 3:i) A feasibility study to produce high specific activity 14C labelled okadaic acid for experimental cell and biochemical research. ii) Production of sufficient purified okadaic acid for tritiation by Amersham International plc. This would require the upgrading of existing HPLC equipment at DML to semi- preparative scale.
Time-Scale and Cost
From: 1993

To: 1993

Cost: £50,000
Contractor / Funded Organisations
Natural Environment Research Council
Fish Disease              
Food Hygiene              
Fields of Study
Fish Health and Aquaculture