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Antioxidants and protein damage in the eye: can diet be optimised for the prevention of cataracts ? - AN0417

Preliminary and very revent clinical studies have shown the low serum antioxidant vitamins are risk factors for cataract formation in humans. No data is available for optimal nutrition for prevention of cataract. In this study, we propose to use cultured human lens cells and intact human lenses under experimentally induced oxidative stress including endogneous agents to determine the effect of (1) nutrient vitamins, (2) non-nutrient antioxidants and (3) endogenous antioxidant enzyme inducers on cataract formation. The latter will be assessed by a combinatin of parameters including intact lens functionality (light scatter and membrane permeability ), determination of lens protein content, aggregation and stability , and measurement of oxidative damage. The results should allow an estimation of the optimal concentration of dietary antioxidants for maintenance of conditions for prevention of cataract.
1. Set up in vitro animal cataract model systems that can be investigated in detail and determine relationship between concentration and lens functionality for each oxidant. 2. Study the ability of nutrient antioxidants to alter the above response. 3. Elucidate possible synergistic interactions between antioxidants. 4. Transfer information from animal cataract models to in vitro human lens systems.
Time-Scale and Cost
From: 1994

To: 1997

Cost: £288,634
Contractor / Funded Organisations
University - East Anglia, IFR - Institute of Food Research (BBSRC)