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Development and Evaluation of a Standard Operating Procedure (SOP) to monitor Antimicrobial Resistance Threats in Freshwater Environments - SD0510

Assessing the levels of antimicrobial agents already in the aquatic environment, and their effect on antibiotic resistance, is an area of research that needs to be given priority (The Royal Society of Chemistry (RSC) evidence to a recent House of Commons Science and Technology Committee’s inquiry into water quality). The proposed study will develop and evaluate methods for assessing levels of antimicrobial resistance (AMR) threat in terms of both contaminant levels and AMR organisms in freshwater environments and link this to relevant inputs (e.g. sewage effluent and agricultural land run-off)
1. Compare the occurrence of antimicrobial resistant bacteria and resistance genes in three different matrices – water, sediment and shellfish to determine their relative sensitivity and applicability to a monitoring program – end Sept 2014.
2. Assess the mechanisms present in microbial communities that drive resistance in freshwater environments end – Nov 2014.
3. Provide an initial indication of the drivers of resistance in freshwater – end Jan 2015.
4. Deliver a standard operating procedure describing sample collection, processing, microbe isolation, resistance testing, molecular assessment of resistance mechanisms and water chemistry
Time-Scale and Cost
From: 2014

To: 2015

Cost: £82,318
Contractor / Funded Organisations