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Development and applications of tests for anti-coagulent resistance in rodents (Joint with PSD - PV0201) (Follow on VC0309) - VC0301

Description
VC0301. Tests for anticoagulant resistance in rodents.

Investigations to establish the geographical distribution of anticoagulant resistance in rodents are dependent on the availability of suitable tests. Rapid blood clotting response tests have been developed for warfarin and difenacoum, but current tests for resistance to other widely used second generation anticoagulants are based on time and resource consuming feeding assays. This study will aim to develop new rapid tests for resistance to anticoagulants in rodents, including, potentially, the use of gene probes. The tests will then be used to determine the geographical distribution of anticoagulant resistant rodents in England and Wales. Effects of bromadiolone will be evaluated on blood clotting in anticoagulant-susceptible laboratory rats, in order to determine a discriminating dose, which will then be used against anticoagulant-resistant rats. Similar tests will also be conducted for brodifacoum- and flocoumafen-resistance. It is anticipated that the development of such tests will greatly increase the number of samples handled and thus increase knowledge of the distribution of resistance. Use of gene probes may enable considerable insight to be gained into the mode of action of anticoagulants and the mode of action of anticoagulants and the enzymes involved in anticoagulant and vitamin K metabolism.
Objective
To develop new rapid tests for resistance to anticoagulants in rodents, based upon changes in concentration of blood clotting protein. To determine the geographical distribution of anticoagulant resistant rodents in England and Wales. To examine the feasibility of using gene probes for testing for anticoagulant resistance in rodents.
Time-Scale and Cost
From: 1993

To: 1995

Cost: £308,000
Contractor / Funded Organisations
ADAS UK Ltd., Central Science Laboratory
Keywords
              
Fields of Study
Wildlife Management