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Development of Rationally Attenuated live Vaccines for Effective Control of Infectious Bronchitis - OD0720

The overall aim of this project is to attenuate the pathogenicity of infectious bronchitis virus (IBV) of poultry in a non-reversible way, whilst maintaining immunogenicity for both vaccination of chickens and for in ovo application.

Control of infectious diseases and a reduction in the use of therapeutic antibiotics are two major challenges faced by the UK poultry industry. The avian coronavirus, IBV, is a highly contagious poultry pathogen prevalent in all types of poultry flocks worldwide. IBV is the causal agent of infectious bronchitis (IB) and continues to be responsible for economic loss, welfare problems in chickens and a potential risk to food security. IBV preferentially causes respiratory disease, but can also infect other organs such as the kidneys (resulting in kidney disease) or the reproductive tract (resulting in loss of egg production and/or egg quality). IBV has been reported to be responsible for more economic loss to the UK poultry industry than any other infectious disease. Although live attenuated vaccines and inactivated vaccines are universally used in the control of IBV, the protection gained by use of vaccination can be lost either due to vaccine breakdown or the introduction of a new IBV serotype that is not related to the vaccine used, posing a risk to the poultry industry. It is important that new and safer vaccines are developed for the control of IBV. This proposal seeks to develop an infectious clone system for the generation of rationally attenuated IBV vaccines, identifying two spatially distant regions of the genome that can be modified for attenuation. The project is divided into four main objectives:-

1) To produce an IBV reverse genetics system based on the pathogenic M41 strain of IBV.
2) To remove the M41 accessory non-structural genes to identify whether they play a role in pathogenicity.
3) To study the role of the M41 essential (replicase) non-structural protein genes in pathogenicity.
4) To investigate the attenuation of IBV for in ovo vaccination.

This highly innovative project will be carried out by the coronavirus research group at the Institute for Animal Health, Compton which has the necessary IBV reverse genetics technology and the animal facilities to test rIBVs in chickens. The submission is in direct response to research requirements identified and agreed by the Poultry Research Committee at their meeting on the 17th November 2008.
Project Documents
• Other : Development of rationally attenuated live vaccines for the effective control of infectious bronchitis   (3405k)
Time-Scale and Cost
From: 2009

To: 2013

Cost: £405,241
Contractor / Funded Organisations
BBSRC Central Office
Animal Diseases              
Animal Health              
Disease Prevention              
Plants and Animals              
Vaccine Development              
Fields of Study
Animal Health