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Investigation of Staphylococcus aureus isolates from bulk milk which carry a gene homologous to mecA. - OD2030

Description
The proposed study will investigate the possible association between a newly
disovered divergent mecA gene, in a strain of Staphylococcus aureus (S aureus)bacteria, with observed resistance to beta-lactam antibiotics. A mutant strain of the original antibiotic resistant bacteria in which the divergent mecA gene has been deleted will be created. If the divergent mecA gene is responsible for the antibiotic resistance then deletion of the gene should remove the antibiotic resistance.

Additional work will establish the multi-locus sequence types (MLST) of 13 strains of S. aureus that have been identified as carrying the new gene. A phylogenetic analysis of the MLSTs together with other data that is known about these clinical isolates will provide some indication of the possible origin of the divergent mecA gene and provide valuable reference data.
Objective
The proposed work will attempt to complete two tasks:
1. The strain typing of up to24 phenotypically resistant isolates (including the 13 isolates that carry the divergent mecA gene) together with phylogenetic analysis.
2. The production and phenotypic testing of a laboratory generated mutant of S. aureus from which the divergent mecA has been deleted.
Project Documents
• Final Report : Investigation of Staphylococcus aureus isolates from bulk milk which carry a gene homologous to mecA.   (377k)
Time-Scale and Cost
From: 2009

To: 2010

Cost: £52,707
Contractor / Funded Organisations
University - Cambridge
Keywords
Animal Health              
Food Safety              
Milk              
MRSA              
Plants and Animals              
Salmonella              
Zoonoses              
Fields of Study
Animal Health