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Identification of physochemical factors controlling the capacity of nano-particles to penetrate cells of the respiratory epithelium - CB0421

Description
To investigate the mechanisms of particle uptake.
Objective
1) To establish a protocol for transfection of immortalised type I cells with SiRNA and its effect on cell viability;
2) To optimise silencing of genes involved in endocystosis using SiRNA and confirm successful transfection with western blotting;
3) To investigate the effect of gene silencing on uptake of flourescently-labelled latex nanoparticles.
Project Documents
• FRP - Final Report : Identification of physochemical factors controlling the capacity of nano-particles to penetrate cells of the respiratory epithelium   (792k)
Time-Scale and Cost
From: 2008

To: 2009

Cost: £18,412
Contractor / Funded Organisations
Imperial College Consultants Ltd
Keywords
Biotechnology              
Biotech-non GM              
Nanotechnology              
Fields of Study
Chemicals and Nanotechnology