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Development and validation of a multi-residue method for the determination of NSAIDs (including meloxicam and tolfenamic acid) in cattle, pig and horse using liquid chromatography with tandem mass spectrometry. - VM02157

Description
Tolfenamic acid and Meloxicam are both veterinary drugs licensed for use as an anti-inflammatory treatment in cattle and pigs. Meloxicam is also licensed for use in horses. In order to control any drug residues entering the food chain, maximum residue levels (MRLs) are set in European legislation for residues in various edible tissues and in milk.

Currently, UK produce is tested for other NSAIDs including carprofen, vedaprofen and phenylbutazone. It is not tested for tolfenamic acid or meloxicam residues. These MRLs, therefore, are not monitored or enforced.

Analytical methods used to detect veterinary drug residues to check compliance with European legislation must comply with the requirements of Commission Decision 2002/657/EC. This Decision prescribes experimental protocols for determining the performance characteristics of the analytical method, and sets acceptable limits for these performance characteristics.

This proposal is to transfer and optimise a multi-residue method for the analysis of NSAIDs (including meloxicam and tolfenamic acid). This analytical method will then be validated for all required species (cattle, pig and horse). Performance characteristics will be determined and assessed against the requirements of Commission Decision 2002/657/EC, in order that it can be used for checking that residues comply with European legislation.

An inter-laboratory validation of the method will be undertaken by another National Reference Laboratory (Central Science Laboratory in York). It is envisaged that the method will be suitable for both screening of samples and for the confirmation and quantitation of any presumptive residues.
Objective
Objective 1.
Transfer the CRL method to LGC. Optimise the method for the analysis of a range of NSAIDs (including carprofen, flunixin, meloxicam, phenylbutazone, tolfenamic acid and vedaprofen in kidney.

Objective 2
To determine the within-laboratory performance characteristics of the method for the analysis of NSAIDs in cattle kidney as prescribed in Commission Decision 2002/657/EC.

Objective 3.
To determine the inter-species applicability of the method for cattle, pig and horse kidney using a statistical ruggedness test.

Objective 4.
To determine the within-laboratory performance characteristics of the method for the analysis of pig kidney as prescribed in Commission Decision 2002/657/EC. This objective will not be required if no statistically significant difference between species is observed in objective 3.

Objective 5.
To determine the within-laboratory performance characteristics of the method for the analysis of horse kidney as prescribed in Commission Decision 2002/657/EC. This objective will not be required if no statistically significant difference between species is observed in objective 3.

Objective 6.
To determine the between-laboratory performance characteristics of the method for the analysis of kidney (cattle, pig and horse) as prescribed in Commission Decision 2002/657/EC.

Objective 7.
To conduct stability trials of NSAIDs in solution as prescribed in Commission Decision 2002/657/EC.

Objective 8.
To conduct stability trials for cattle kidney fortified with NSAIDs as prescribed in Commission Decision 2002/657/EC.

Objective 9.
To conduct stability trials for pig kidney fortified with NSAIDs as prescribed in Commission Decision 2002/657/EC.

Objective 10.
To conduct stability trials for horse kidney fortified with NSAIDs as prescribed in Commission Decision 2002/657/EC.
Time-Scale and Cost
From: 2007

To: 2008

Cost: £97,813
Contractor / Funded Organisations
LGC
Keywords
Animal Health              
Method Development              
NSAID              
Plants and Animals              
Residues              
Residues Testing              
Veterinary Medicines              
Fields of Study
Animal Health
Veterinary Medicine