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Detailed characterisation of novel phage antibodies that bind the amino terminus of the prion protein - SE2009

Description
Previous defra funding (SE1765) has enabled the selection of four new single chain antibodies (scFvs) that specifically recognise the N-terminal domain of PrPc. The new scFvs are novel in that they recognise the central region of the prion protein, a region of documented biological and diagnostic importance and display binding profiles by ELISA and western blot that are different to several existing monoclonal antibodies. Their
novelty is such that the main objective of this proposal is to further investigate their binding characteristics through reconstruction into full length antibodies for assessment of diagnostic potential. A secondary
objective to isolate further antibodies using the techniques established and proven with the initial award. The work is clearly relevant to policy as indicated by in the current TSE Research Requirements Document
(TSE RRD 2005) specific points R5 and R6. The intended use of the results is to further understand these novel agents of disease and to aid the development of sensitive and rapid diagnostic tests that can be used to
detect TSE infection in live animals.
Project Documents
• Final Report : Detailed characterisation of novel phage antibodies that bind the amino terminus of the prion protein   (961k)
• Executive Summary : Detailed characterisation of novel phage antibodies that bind the amino terminus of the prion protein   (146k)
Time-Scale and Cost
From: 2006

To: 2008

Cost: £179,543
Contractor / Funded Organisations
University - Reading
Keywords
Animal Health              
Antibody              
Diagnosis              
Plants and Animals              
TSE              
TSEs