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Understanding gene expression changes in the rosy apple aphid on different hosts as a means to seek new control strategies - HH3125STF

Description
§ Aphids are some of the most serious pests of cultivated plants. During the course of evolution, plants have evolved a vast array of defence strategies to counteract aphid attack. Apple breeders have been able to identify such preadaptive material in Malus and use it to breed apple varieties resistant to two apple aphid species (rosy leaf curling aphid and woolly aphid). For the rosy apple aphid, the most serious aphid pest on apple, however, it has proven difficult to identify sources of natural resistance. This aphid is therefore controlled entirely by spraying insecticides. Because of the limited number of aphidicides compatible with IPM and the high risk of resistance to such pesticides, we propose to explore novel methodologies to efficiently seek out differential host effects on the rosy apple aphid as a means to identify future potential control resources

Specifically we will use new gene expression profiling technology to:
§ monitor the transcriptional background leading up to the aphid leaving its primary apple host and temporarily moving to its secondary host plantain. By characterising genes turned on or off during this time, we expect to gain a novel insight into what is inducing and adapting them to leave apple.
Objective
1: Determine changes in gene expression profile and characterise genes involved 1) during the period on apple leading up to the aphid leaving for its secondary host, and 2)in adapting the aphid to survive on two different hosts during the annual cycle.

2: Determine differences in gene expression profile and characterise genes involved when RAA is reared on apple cultivars of high and low susceptibility.

3: Interpret the physiological underpinning to differential gene expression during host alternation and on alternative cultivar hosts, as a potential means to identifying future exploitable control possibilities.
Project Documents
• Final Report : Understanding gene expression changes in the rosy apple aphid on different hosts as a means to seek new control strategies   (393k)
Time-Scale and Cost
From: 2005

To: 2008

Cost: £172,066
Contractor / Funded Organisations
East Malling Research
Keywords
Allocated - EMR              
Apples              
Biological Control              
Biotechnology              
Farming              
Fruit              
Horticulture              
Pest Control              
Sustainable Farming and Food              
Sustainable Production              
Fields of Study
Horticulture
Horticulture