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Comparison of Electrophoretic and Botanical methods of Authentication of VCU Seed Submissions - VS02005


The overall objective will be to compare directly the results of authentication of VCU stocks using protein electrophoresis techniques with those obtained by comparing the botanical characters of plots grown from VCU and DUS seed.

The project will provide data which will inform objectively the current proposal to use electrophoresis as a method for VCU authentication in future.

Electrophoresis data on all NL1 and NL2 candidate varieties of Wheat, Barley, Oats and Triticale will be acquired. These data will comprise patterns from both the DUS and VCU submissions for the 2001/2002 trialling season.

Authentication provided using electrophoresis with that obtained in parallel from observation plots will be compared. Highlight points of agreement and disagreement.

Points of disagreement will be investigated by additional electrophoresis testing and, if time permits, by more detailed field observation.

The project will provide an objective assessment of the effect on the robustness of VCU authentication of a change from ‘observation only’ to ‘electrophoresis only’. Four possible conclusions are predicted:

a) Visual assessment reveals a sub set of the authentication anomalies found by electrophoresis.
b) Electrophoresis reveals a sub-set of the authentication anomalies found by visual assessment.
c) Both electrophoresis and visual assessment find identical authenication anomalies.
d) Some authentication anomalies are identified by both visual and electrophoretic methods, others are identified by only one or other method.

It is likely that the conclusions will be slightly different for each of the species examined. Conclusion (d) is believed to be the most likely conclusion overall.

Project Documents
• Final Report : Comparison of Electrophoretic and Botanical methods of Authentication of VCU Seed Submissions   (31k)
Time-Scale and Cost
From: 2002

To: 2003

Cost: £10,000
Contractor / Funded Organisations
National Institute of Agricultural Botany
Arable Farming              
Plant Varieties and Seeds              
Plants and Animals              
Variety Testing              
Fields of Study
Plant Varieties and Seeds