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Plant Health Fellowship: (1) Development of molecular techniques to distinguish vegetative compatibility groups in fungi. - PH0302

Description
First Plant Health Fellowship, aim to address lack of taxonomic expertise, implications for succession planning for diagnostics which is an essential part of the UK PH Service.

This will be a one year study at CSL looking at the development of molecular techniques to to discriminate fungal pathogens at and below the species level.

Future fellowships will be for 3 year PhD studies.

Purpose
The diagnosis and detection of plant pests and pathogens of statutory concern is a basic and integral part of Defra Plant Health policy. Improvements in diagnostic techniques are continually sought to extend current capabilities. Recently the adoption of novel diagnostic techniques have made an impact in this area, for example PCR techniques (including those based on TaqMan) are well established for viral, bacterial and fungal pathogens, and are becoming available for invertebrate pests. The use of these techniques is primarily limited by the availability of appropriate sequence information from which to design the PCR reagents.

Using Rhizoctonia solani as a model system the aims of the project are as follows –

1. To investigate the potential utility of COX subunit sequences for the ability to discriminate at and below the species level.
2. To compare the discrimination at and below the species level obtained with COX sequences with currently available ITS sequences.
Objective
1. To investigate the suitability of COX subunit nucleotide sequence for the discrimination of Rhizoctonia species and sub species (AGs) and to compare the clustering achieved at the species and sub-species level with that achieved using ITS nucleotide sequence.
Project Documents
• Final Report : Plant Health: Development of molecular techniques to distinguish vegetative compatibility groups in fungi: Rhizoctonia spp. a case study (Plant Health Fellowship 1)   (204k)
Time-Scale and Cost
From: 2004

To: 2005

Cost: £15,000
Contractor / Funded Organisations
Central Science Laboratory
Keywords
Diagnosis              
Molecular Biology              
Plant diseases              
Plant health              
Plants and Animals              
Fields of Study
Plant Health