Defra - Department for Environment, Food and Rural Affairs.

Science Search

Science and Research Projects

Return to Science Search homepage   Return to Project List

A physical map of the pig genome as a launch pad for finding genes of economic importance - LS3105

A physical cloned based map of the pig genome will provide a launch pad for finding genes of economic importance and for developing molecular tools for marker assisted selection. Large fragment genomic clones in a BAC library will be characterised by DNA fingerprinting. From analysis of DNA fingerprints from ca. 100,000 independent clones the overlap between different clones can be established. From these overlapping relationship contig maps of the geneome can be deduced. Genetic markers developed from, or mapped to the clones provide a link to quantitative trait loci (QTL) mapped in experimental and commercial populations. Thus, clones that can be assigned to QTL can be exploited in the search for the genetic lesions underlying QTL and for the development of more effective genetic markers for marker assisted selection.
1. To acquire copies of the Roslin pigE BAC and CHORI-242 BAC libraries
2. To establish working and archive copies of these two BAC libraries
3. To characterise ca. 100,000 BAC clones by DNA fingerprinting
4. To construct BAC contig physical maps of the pig genome
5. To integrate the physical map data with other groups in order to develop fully annotated comprehensive physical maps of the pig genome.
Time-Scale and Cost
From: 2003

To: 2004

Cost: £150,000
Contractor / Funded Organisations
Roslin Institute, Edinburgh (BBSRC)
Biotech-non GM              
Livestock Farming              
Meat Quality