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The development and validation of a quantitative analysis method for phenlybutazone in horse serum - VM02138

Objective :

1). To synthesise a deuterated analogue of phenybutazone to act as internal marker for the quantitative assay;
2). To develop a quantitative LC-MS method for phenylbutazone with a lower limit of quantification of 25 ng/ml in horse serum;
3). To validate the method according to the SANCO Guidelines; and
4). To provide appropriate training documentation and materials to UK laboratories involved in the statutory and non-statutory schemes for testing for veterinary residues.


To enable to UK Surveillance programme to comply with EC Regulations.

Intended Use:

An analytical strategy for the regulatory control of phenylbutazone as part of the National Surveillance Programme.
Project Documents
• Final Report : The development and validation of a quantitative analysis method for phenylbutazone in horse serum   (337k)
Time-Scale and Cost
From: 2003

To: 2003

Cost: £18,540
Contractor / Funded Organisations
HFL Limited
Animal Health              
Method Development              
Plants and Animals              
Residues Testing              
Screening Assays              
Veterinary Medicines              
Fields of Study
Animal Health
Veterinary Medicine