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Detection of illegal or unauthorised hormone use (HORMONE RADAR) - VM02135

Description
This project is submitted in response to DEFRA’s call for proposals to develop procedures for the detection of illegal and unauthorised hormone use.

The first objective of this project proposal is to develop rapid in vitro screening assay(s) methodology capable of detecting the administration of illegal or unauthorised hormonal growth promoting veterinary drugs. This will be achieved by the development of novel procedures based on a variety of receptor assays.

The second objective is to develop validated confirmatory methods that will detect and confirm which drugs have been detected by the screening assays. These confirmatory methods will be based on techniques such as LCMS/MS and TOF/MS.

As part of this project proposal the developed techniques will be made transferable to other competent laboratories. In addition the methodology will be validated to European standards by the use of incurred samples.

In this proposal three EU National Reference laboratories will work together to ensure that, over the life of this project, the focus of the project remains relevant to both UK and Pan-European issues as they arise.
Objective
Objective 1. To develop generic and specific extraction and clean-up protocols appropriate to the screening and identification assays to be developed. (CSL/VSD) Objective 2. To develop/produce receptors, receptor fragments and anti-receptor antibodies capable of use within the following receptor-based screening assays (in order of suggested priority) (i) Beta-agonists (ii) androgens (iii) glucocorticoids and (iv) oestrogens. (VSD) Objective 3. To develop high-sensitivity mass spectrometry methodology for the identification of unknowns likely to be detected as a result of objective 2. (CSL/RIKILT) Objective 4. To develop methodology for the detection of estrogenic, androgenic, ß-agonist, and corticosteroid activity in animal tissues and identification of the causative agent. (CSL/VSD) Objective 5. To validate the developed methodology against a range of compounds.
Project Documents
• Final Report : Detection of illegal or unauthorised hormone use (HORMONE RADAR)   (999k)
• Final Report : Detection of illegal or unauthorised hormone use (HORMONE RADAR)   (24k)
• Final Report - Annex : Detection of illegal or unauthorised hormone use (HORMONE RADAR)   (641k)
• Final Report - Annex : Detection of illegal or unauthorised hormone use (HORMONE RADAR)   (23k)
• Final Report - Annex : Detection of illegal or unauthorised hormone use (HORMONE RADAR)   (23k)
Time-Scale and Cost
From: 2002

To: 2006

Cost: £392,146
Contractor / Funded Organisations
University - Belfast - Queens, RIKILT, Central Science Laboratory
Keywords
Animal Health              
Biotechnology              
GM Non-Food              
Method Development              
Plants and Animals              
Residues              
Residues Testing              
Screening Assays              
Veterinary Medicines              
Fields of Study
Animal Health
Veterinary Medicine