Defra - Department for Environment, Food and Rural Affairs.

Science Search

Science and Research Projects

Return to Science Search homepage   Return to Project List

Smart plant technology for sensing crop nutritional status - AR0911

Description
Abstract of Research Proposal

The project aims to identify plant genes whose expression is regulated by specific nutrient status, particularly by N and S. Genes will be evaluated for quantitative expression in relation to differential nutrient status in wheat. Promoters of these genes will be isolated and coupled to reporter genes and used in a transient expression assay system to evaluate responsiveness to nutritional status following re-introduction by bombardment. The promoters from this screening programme coupled to appropriate reporter genes (fluourescence, luminescence) will be suitable for introduction into transgenic crops or specific sentinel plants which are able to signal or communicate the nutritional status of the crop. Field-based detection would be through hand-held or tractor-based monitoring. This ‘smart plant’ technology will aid precision farming, avoiding uneconomic and potentially environmentally damaging excessive application of fertilisers.

Summary of Objectives

1. Identification and isolation of genes regulated by specific nutrient status using cDNA-AFLPs.
2. Examine robustness of expression and dose responsiveness with respect to applied fertiliser levels and quantified nutritional status.
3. Isolation of regulatory elements (promoters) responsible for nutrition regulation of gene expression, preparation of promoter-reporter constructs and use in transient assay analysis to assess effectiveness of reporting systems following re-introduction into plant tissues.




Funding Information
£210,000

Start date 1 December 2001
Completion date 30 November 2004


Objective
1. Identification and isolation of genes regulated by specific nutrient status using cDNA-AFLPs
2. Examine robustness of expression and dose responsiveness with respect to applied fertiliser levels and quantified nutritional status
3. Isolation of regulatory elements (promoters) responsible for nutrition regulation of gene expression, preparation of promoter-reporter constructs and use in transient assay analysis to assess effectiveness of reporting systems following re-introduction into plant tissues
Project Documents
• Final Report : Smart plant technology for sensing crop nutritional status   (5890k)
Time-Scale and Cost
From: 2001

To: 2004

Cost: £209,608
Contractor / Funded Organisations
Rothamsted Research (BBSRC)
Keywords
Arable Farming              
Biotechnology              
Crop Improvement              
Crops              
Farming              
GM Food              
Sustainable Farming and Food              
Sustainable Production              
Fields of Study
Arable Crops