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Mushroom quality-loss after harvest - causes and control - HH2116SMU

Description
Quality loss processes in the mushroom postharvest are under genetic control. The library of genes up-regulated after harvest will be characterised so that control strategies to improve mushroom quality can be developed and assessed. The transcript levels of five genes will be determined showing when after harvest and in which tissue transcription occurs and the abundancy of the transcript. Agronomic control strategy will be tested by stressing a crop prior to harvest and examining pre-adaptation to harvesting stress by determining superoxide dismutase transcript levels. A second control strategy, breeding, will be investigated by examining the effects of silencing one of the up-regulated genes.
Objective
The overall objective is to understand the genetic, biochemical and physiological factors controlling postharvest quality-loss in mushrooms and to use this knowledge to control this process.

1. To examine patterns of transcription (spatial and temporal) of five remaining genes of known function in the cDNA library.

2. To determine whether environmental stresses applied prior to harvest can stimulate changes in the postharvest biology of the mushroom by stimulating an increase in superoxide dismutase transcript levels.

3. To downregulate transcript level of one specific gene using transformation and anti-sense technologies and determine effect on postharvest biology of the sporophore.
Project Documents
• Final Report : Mushroom quality-loss after harvest - causes and control   (746k)
Time-Scale and Cost
From: 2001

To: 2005

Cost: £397,220
Contractor / Funded Organisations
Warwick - HRI, Horticulture Research International
Keywords
Biotechnology              
Crop Improvement              
Farming              
GM Food              
Horticulture              
Mushrooms              
Quality              
Sustainable Farming and Food              
Sustainable Production              
Vegetables              
Fields of Study
Horticulture
Horticulture