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Do Strains of Mouse Adapted Scrapie and BSE Correlate with Strains seen in the natural host - SE1440

Description
To establish whether mouse adapted TSE strains correlate with strains in the natural host it is proposed to passage BSE and scrapie through wild type mice and transgenic mice carrying and expressing different polymorphic variants of the PrP gene of sheep (scrapie and BSE) and cattle (BSE) and compare the strain characteristics in the wild type and transgenic mice with those resulting from a further passage through wild type mice (see plan of protocol in diagram 1a and 1b and 1c).

This will involve:

·Passage of cattle BSE through wild type mice with different PrP genotypes to define incubation time, vacuolar pathology and PrPSc distribution and PrP glycoforms in these mice

·Passage of cattle BSE and mouse passaged BSE through bovine transgenic mice to define incubation time, vacuolar pathology and PrPSc distribution and PrP glycoforms in these mice

·Subpassage of infectious material from BSE infected bovine transgenic mice to C5lBL, VM and RIII mice to compare the strain characteristics with first and second passage of cattle BSE

·Passage of natural scrapie through wild type mice with different PrP genotypes to define incubation time, vacuolar pathology and PrPSc distribution and PrP glycoforms in these mice

·Passage of natural scrapie and mouse passaged scrapie through sheep transgenic mice to define incubation time, vacuolar pathology and PrPSc distribution and PrP glycoforms in these mice

·Subpassage of infectious material from scrapie infected sheep transgenic mice to C57BL, VM and RIII mice to compare the strain characteristics with first and second passage of cattle BSE

·Passage of BSE through sheep transgenic mice and subpassage of infectious material from BSE infected sheep transgenic mice through C57BL,VM and RIII mice,and compare strain characteristics with passage through bovine transgenics

This will establish the extent to which the endogenous PrP gene of the infected animal and other factors may modify the strain characteristics of the emerging infectious agent. It will address whether BSE passaged through sheep will retain the strain characteristics of BSE. This directly addresses MAFF TSERRD 2.1.
Project Documents
• Final Report : Do strains of mouse adapted scrapie and BSE correlate with strains seen in the natural host?   (1061k)
Time-Scale and Cost
From: 2001

To: 2007

Cost: £1,260,900
Contractor / Funded Organisations
Institute for Animal Health (BBSRC)
Keywords
Animal Health              
BSE              
Plants and Animals              
Scrapie              
Sheep              
Strain              
Transmission              
TSEs              
Fields of Study
Animal Health