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An investigation into strategies to improve pea transformation - AR1004

The routine transformation of Pisum sativum is an essential component of the study of gene function for crop improvement in this important legume. Pea transformation has been established in several laboratories in Europe and the behaviour of a number of genes studied as a direct result of a previous MAFF-funded project (ARO130). However there are several drawbacks to established procedure that could, if overcome, result in a much improved protocol. This proposal seeks to build on the established methodology and address two of the problems that hamper routine transformation: 1. Investigation of kanamycin resistance genes as an alternative to bar as a selection gene and 2. Investigation of alternative novel methods for legume transformation (reported for a model legume) that, if proven applicable to pea, could generate larger numbers of transformants with greater efficiency. The knowledge gained from this work will be applied to a range of projects that seek to define genes involved in an array of agronomically useful characters.
1. To investigate kanamycin as an alternative selection system for transformed peas

2. To investigate the transformation procedures in place for M. truncatula and to adapt these for use in pea

3. To provide information on transgene expression in meristematic cells and to correlate this with transgene inheritance in seeds from cloned shoots.
Project Documents
• Final Report : An investigation into strategies to improve pea transformation   (5437k)
Time-Scale and Cost
From: 2001

To: 2003

Cost: £73,405
Contractor / Funded Organisations
John Innes Centre (BBSRC)
Arable Farming              
Crop Improvement              
GM Food              
Fields of Study
Arable Crops