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Development of a novel FRET-based assay for the detection of PrPSc in various biological matrices - SE1768

Description
The need for a highly sensitive sub-clinical test is of paramount importance to the eradication of TSEs and ensuring that infective tissue does not enter the food supply. Early detection using a readily available biological matrix such as blood or lymphoreticular tissue of sheep would allow infection to be determined, before clinical signs and suffering of the animal occurs.Extensive surveillance and monitoring for sub clinical detection of TSEs, in particular BSE, stands to play a major role in future aims to eradicate the disease and then to ensure that the disease remains absent from the national herd. Surveillance on such a large scale and of such an important pathogen requires not only a highly sensitive and specific test, but a test that is cost effective, easily executed at a technical level, safe for the operator and rapid (amenable to high throughput processing).

The main objective of this study is to establish proof of principal and undertake basic optimisation of a novel immuno-FRET diagnostic test for the detection of PrPSc in various matrices. Optimisation will focus on ease and speed of use and maximising specificity and sensitivity. One major advantage of the FRET-based test described here is that the change in fluorescent signal only occurs when the labelled antibodies are bound to the specific target, therefore non-bound antibodies do not need to be removed from the assay. The assay proposed here is therefore an homogeneous assay that significantly improves the rapidity and safety of processing.

This revision is an extension of milestones with no added funding necessary.
Project Documents
• Final Report : Development of a novel FRET-based assay for the detection of PrPsc in various biological matrices   (101k)
• Final Report : Development of a novel FRET-based assay for the detection of PrPsc in various biological matrices   (101k)
Time-Scale and Cost
From: 2001

To: 2003

Cost: £233,721
Contractor / Funded Organisations
Veterinary Laboratories Agency
Keywords
Animal Health              
Antibody              
Diagnosis              
FRET              
Plants and Animals              
TSE              
TSEs              
Fields of Study
Animal Health