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Epidemiology of new disease problems including mushroom virus x - HH2304SMU

This project aims to determine the relationships between the dsRNA bands consistently found in Agaricus bisporus displaying symptoms of mushroom virus X (MVX). This will be achieved by sequencing the 3’ end of all major bands available for analysis by terminal end labelling techniques. Sequence comparison will enable bands to be grouped and from this it will be possible to identify target molecules to develop a PCR diagnostic test. Antibodies raised to A. bisporus in HH2304SPC will be assessed for cross reactivity with other fungi commonly found on mushroom farms. The ability of antisera to detect A. bisporus on farm using MTIST will be assessed. Experiments will be conducted to determine whether dsRNA elements can be transmitted between strains and to assess the effects of cross-infection between two strains with different dsRNA profiles. This is crucial to our understanding of movement of MVX through the mushroom industry.
This work addresses the policy objective ‘ to provide robust strategies and methods of disease control suitable for integrated crop and farm management’
1. Carry out statistical analysis of existing data set to determine relationship between dsRNA bands.

2. To isolate and clone at least 8 dsRNA molecules associated with mushroom virus X disease and determine the relationship between cloned bands.

3. To sequence at least 400 bases of each of at least 8 dsRNA bands to determine their relationship with published virus sequences.

4. Determine dsRNA profile from 40 single spore cultures.

5. To develop a PCR test for at least 3 discrete viruses.

6. Investigate use of selective media and antibody detection for in field dectection of Agaricus.
Project Documents
• Final Report : Epidemiology of new disease problems including mushroom virus x   (2089k)
Time-Scale and Cost
From: 2001

To: 2002

Cost: £236,549
Contractor / Funded Organisations
Horticulture Research International
Disease Control              
Fields of Study